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KMID : 0370220060500060409
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Yakhak Hoeji
2006 Volume.50 No. 6 p.409 ~ p.414
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Alteration of Cytosolic Ca+2 Signal by Cryopreservation in pig Sperm
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Lee Sun-woo
Park Chang-Sik
Myung Pyung-Keun
Li Yu-hua
Kim Joon-Chul
Woo Sun-Hee
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Abstract
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Although mammalian sperms are cryopreserved for in vitro tertilization a process of cryopreservation decreases the feruility . Acrosome reaction requires depolarization-induced Ca2+ influx and Ca2+ releases from the Ca2+stores. To examine whether the cellular Ca2+ mobilization is altered by a sperm cryopreservation we compared cytosolic Ca2+ signals between fresh and cryopreserved pig sperms using confocal Ca2+imaging. The magnitudes of depolarization-induced Ca2+increases were significantly smaller in cryopreservedsperms. Exposures to 10mM caffeine or 5μM thapsigargin elicited less Ca2+increases in th cryopreserved sperms compared to fresh sperms. In addition, progesterone-triggered Ca2+rises, that are thought to enhance acrosome reaction, werecompletely abolished in the cryopreserved sperms. These results suggest that storage and (/or) release of Ca2+ from the intracellular Ca2+from the intracellular Ca2+stores in pig sperms are significantly impaired by the process of cryopreservation.
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KEYWORD
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pig sperm, cryopreservation, cytosolic Ca+2
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